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Biofilms will form on virtually every non-shedding surface in a non-sterile aqueous (or very humid) environment.
- Discuss the importance of biofilms in the biomedical community
- Biofilms have been found to be involved in a wide variety of microbial infections in the body.
- Microbes form a biofilm in response to many factors, which may include cellular recognition of specific or non-specific attachment sites on a surface and nutritional cues.
- Bacterial biofilms may impair cutaneous wound healing and reduce topical antibacterial efficiency in healing or treating infected skin wounds.
- biofilm: A thin film of mucus created by and containing a colony of bacteria and other microorganisms.
- sterile: unable to reproduce (or procreate)
A biofilm is an aggregate of microorganisms in which cells adhere to each other on a surface. These adherent cells are frequently embedded within a self-produced matrix of extracellular polymeric substance (EPS).
Microbes form a biofilm in response to many factors, which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics. When a cell switches to the biofilm mode of growth, it undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated.
Biofilms are ubiquitous. Nearly every species of microorganism, not only bacteria and archaea, have mechanisms by which they can adhere to surfaces and to each other. Biofilms will form on virtually every non-shedding surface in a non-sterile aqueous (or very humid) environment.
Biofilms have been found to be involved in a wide variety of microbial infections in the body, by one estimate in 80% of all infections. Infectious processes in which biofilms have been implicated include common problems such as urinary tract infections, catheter infections, middle-ear infections, formation of dental plaque, gingivitis, and coating contact lenses. Biofilms have also been implicated in less common but more lethal processes such as endocarditis, infections in cystic fibrosis, and infections of permanent indwelling devices such as joint prostheses and heart valves.
More recently it has been noted that bacterial biofilms may impair cutaneous wound healing and reduce topical antibacterial efficiency in healing or treating infected skin wounds. It has recently been shown that biofilms are present on the removed tissue of 80% of patients undergoing surgery for chronic sinusitis. The patients with biofilms were shown to have been denuded of cilia and goblet cells, unlike the controls without biofilms who had normal cilia and goblet cell morphology. Biofilms were also found on samples from two of 10 healthy controls mentioned. The species of bacteria from interoperative cultures did not correspond to the bacteria species in the biofilm on the respective patient’s tissue. In other words, the cultures were negative though the bacteria were present.
Biofilms can also be formed on the inert surfaces of implanted devices such as catheters, prosthetic cardiac valves, and intrauterine devices. New staining techniques are being developed to differentiate bacterial cells growing in living animals, e.g. from tissues with allergy-inflammations.
Pseudomonas aeruginosa biofilms
The achievements of medical care in industrialized societies are markedly impaired due to chronic opportunistic infections that have become increasingly apparent in immunocompromised patients and the aging population. Chronic infections remain a major challenge for the medical profession and are of great economic relevance because traditional antibiotic therapy is usually not sufficient to eradicate these infections.
Pseudomonas aeruginosa is not only an important opportunistic pathogen and causative agent of emerging nosocomial infections but can also be considered a model organism for the study of diverse bacterial mechanisms that contribute to bacterial persistence. In this context the elucidation of the molecular mechanisms responsible for the switch from planktonic growth to a biofilm phenotype and the role of inter-bacterial communication in persistent disease should provide new insights. It should help researchers learn about the pathogenicity of P. aeruginosa, contribute to a better clinical management of chronically infected patients, and lead to the identification of new drug targets for the development of alternative anti-infective treatment strategies.
Dental plaque is a biofilm that adheres to teeth surfaces and consists of bacterial cells, salivary polymers, and bacterial extracellular products. This accumulation of microorganisms subject the teeth and gingival tissues to high concentrations of bacterial metabolites which results in dental disease. The biofilms attached to the surfaces of some dental alloys, impression materials, dental implants, restorative and cement materials play an essential role concerning the biofilms establishment dynamics toward the physical-chemical properties of the materials which biofilms are attached to.
Legionella bacteria are known to grow under certain conditions in biofilms, in which they are protected against disinfectants. Workers in cooling towers, persons working in air conditioned rooms, and people taking a shower are exposed to Legionella by inhalation when the systems are not well designed, constructed, or maintained. Neisseria gonorrhoeae is an exclusive human pathogen. Recent studies have demonstrated that it utilizes two distinct mechanisms for entry into human urethral and cervical epithelial cells involving different bacterial surface ligands and host receptors. In addition, it has been demonstrated that the gonococcus can form biofilms on glass surfaces and over human cells. There is evidence for the formation of gonococcal biofilms on human cervical epithelial cells during natural disease. Evidence also suggests that the outer membrane blebbing by the gonococcus is crucial in biofilm formation over human cervical epithelial cells.
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Biofilm, aggregate of bacteria held together by a mucuslike matrix of carbohydrate that adheres to a surface. Biofilms can form on the surfaces of liquids, solids, and living tissues, such as those of animals and plants. Organisms in biofilms often display substantially different properties from the same organism in the individual, or free-living (planktonic), state. Communities form when individual organisms, which may be of the same or different species, adhere to and accumulate on a surface this process is called adsorption. Following a period of growth and reproduction, the organisms produce an extracellular matrix consisting of carbohydrates called polysaccharides. This matrix serves to hold the bacteria together and to irreversibly bind them to the surface.
Bacteria that have aggregated into biofilms can communicate information about population size and metabolic state. This type of communication is called quorum sensing and operates by the production of small molecules called autoinducers, or pheromones. The concentration of quorum-sensing molecules—most commonly peptides or acylated homoserine lactones (AHLs special signaling chemicals)—is related to the number of bacteria of the same or different species that are in the biofilm and helps coordinate the behaviour of the biofilm.
Biofilms are advantageous to bacteria because they provide a nutrient-rich environment that facilitates growth and because they confer resistance to antibiotics. Biofilms can cause severe infections in hospitalized patients the formation of biofilms in these instances is typically associated with the introduction into the body of foreign substrates, such as artificial implants and urinary catheters. Biofilms also form on the thin films of plaque found on teeth, where they ferment sugars and starches into acids, causing the destruction of tooth enamel. In the environment, biofilms fill an important role in the breakdown of organic wastes by filtering wastes from water and by removing or neutralizing contaminants in soil. As a result, biofilms are used to purify water in water treatment plants and to detoxify contaminated areas of the environment.
This article was most recently revised and updated by Robert Lewis, Assistant Editor.
How fatal biofilms form
By severely curtailing the effects of antibiotics, the formation of organized communities of bacterial cells known as biofilms can be deadly during surgeries and in urinary tract infections. Yale researchers have just come a lot closer to understanding how these biofilms develop, and potentially how to stop them.
Biofilms form when bacterial cells gather and develop structures that bond them in a gooey substance. This glue can protect the cells from the outside world and allow them to form complex quasi-organisms. Biofilms can be found almost everywhere, including unwashed shower stalls or the surfaces of lakes. Because the protective shell can keep out potential treatments, biofilms are at their most dangerous when they invade human cells or form on sutures and catheters used in surgeries. In American hospitals alone, thousands of deaths are attributed to biofilm-related surgical site infections and urinary tract infections.
"Biofilms are a huge medical problem because they are something that makes bacterial infections very difficult to deal with," said Andre Levchenko, senior author of the study, which was published Oct. 5 in Nature Communications.
Fighting biofilms has been particularly difficult because it hasn't been well understood how bacteria cells make the transition from behaving individually to existing in collective structures. However, the researchers in the Levchenko lab, working with colleagues at the University of California-San Diego, recently found a key mechanism for biofilm formation that also provides a way to study this process in a controlled and reproducible way.
The investigators designed and built microfluidic devices and novel gels that housed uropathogenic E. coli cells, which are often the cause of urinary tract infections. These devices mimicked the environment inside human cells that host the invading bacteria during infections. The scientists found that the bacterial colonies would grow to the point where they would be squeezed by either the walls of the chamber, the fibers, or the gel. This self-generated stress was itself a trigger of the biofilm formation.
"This was very surprising, but we saw all the things you would expect from a biofilm," said Levchenko, the John C. Malone Professor of Biomedical Engineering and director of the Yale Systems Biology Institute. "The cells produced the biofilm components and suddenly became very antibiotic-resistant. And all of that was accompanied by an indication that the cells were under biological stress and the stress was coming from this mechanical interaction with the environment."
With this discovery, Levchenko said, researchers can use various devices that mimic other cellular environments and explore biofilm formation under countless environments and circumstances. They can also use the devices introduced in this study to produce biofilms rapidly, precisely, and in high numbers in a simple, inexpensive, and reproducible way. This would allow screening drugs that could potentially breach the protective layer of the biofilms and break it down.
"Having a disease model like this is a must when you want to do these kinds of drug-screening experiments," he said. "We can now grow biofilms in specific shapes and specific locations in a completely predictable way."
Attacking biofilms that cause chronic infections
A clever new imaging technique discovered at the University of California, Berkeley, reveals a possible plan of attack for many bacterial diseases, such as cholera, lung infections in cystic fibrosis patients and even chronic sinusitis, that form biofilms that make them resistant to antibiotics.
By devising a new fluorescent labeling strategy and employing super-resolution light microscopy, the researchers were able to examine the structure of sticky plaques called bacterial biofilms that make these infections so tenacious. They also identified genetic targets for potential drugs that could break up the bacterial community and expose the bugs to the killing power of antibiotics.
"Eventually, we want to make these bugs homeless," said lead researcher Veysel Berk, a postdoctoral fellow in the Department of Physics and the California Institute for Quantitative Biosciences (QB3) at UC Berkeley.
Berk and his co-authors, including Nobel laureate and former UC Berkeley professor Steven Chu, report their findings in the July 13 issue of the journal Science.
"In their natural habitat, 99.9 percent of all bacteria live as a community and attach to surfaces as biofilms according to the National Institutes of Health, 80 percent of all infections in humans are related to biofilms," Berk said.
The researchers were able to employ new techniques that allowed them to zoom into a street-level view of these biofilms, where they learned "how they grow from a single cell and come together to form rooms and whole buildings," Berk said. "Now, we can come up with a logical approach to discovering how to take down their building, or prevent them from forming the building itself."
Combining super-resolution microscopy with the technique Berk developed, which allows continuous labeling of growing and dividing cells in culture, biologists in many fields will be able to record stop-motion video of "how bacteria build their castles," he said.
"This work has led to new insights into the development of these complex structures and will no doubt pave the way to new approaches to fighting infectious disease and also bacteriological applications in environmental and industrial settings," said Chu, a former UC Berkeley professor of physics and of molecular and cell biology and former director of the Lawrence Berkeley National Laboratory.
Bacteria are not loners
The popular view of bacteria is that they are free-living organisms easily kept in check by antibiotics, Berk said. But scientists now realize that bacteria spend most of their lives in colonies or biofilms, even in the human body. While single bacteria may be susceptible to antibiotics, the films can be 1,000 times more resistant and most can only be removed surgically.
Implants, such as pacemakers, stents and artificial joints, occasionally become infected by bacteria that form biofilms. These biofilm sites periodically shed bacteria -- adventurers, Berk calls them -- which can ignite acute infections and fever. While antibiotics can knock out these free-swimming bacteria and temporally calm down the infection, the biofilm remains untouched.The only permanent solution is removal of the biofilm-coated device and replacement with a new sterilized implant.
A permanent bacterial biofilm in the sinuses can ignite an immune response leading to chronic sinus infections, with symptoms including fever and cold-like symptoms. So far, the most effective treatment is to surgically remove the affected tissue.
Bacteria also form permanent, mostly lifelong, biofilms in the mucus-filled lungs of cystic fibrosis patients and are responsible for the chronic lung infections that lead to early death. Although long-lasting antibiotic treatment helps, it cannot eradicate the infection completely.
To study a biofilm formed by cholera bacteria (Vibrio cholerae), Berk built his own super-resolution microscope in the basement of UC Berkeley's Stanley Hall based on a 2007 design by coauthor Xiaowei Zhuang, Chu's former post-doctoral student who is now a professor at Harvard University. To actually see these cells as they divided to form "castles," Berk devised a new technique called continuous immunostaining that allowed him to track four separate target molecules by means of four separate fluorescent dyes.
He discovered that, over a period of about six hours, a single bacterium laid down a glue to attach itself to a surface, then divided into daughter cells, making certain to cement each daughter to itself before splitting in two. The daughters continued to divide until they formed a cluster -- like a brick and mortar building -- at which point the bacteria secreted a protein that encased the cluster like the shell of a building.
The clusters are separated by microchannels that may allow nutrients in and waste out, Berk said.
"If we can find a drug to get rid of the glue protein, we can move the building as a whole. Or if we can get rid of the cement protein, we can dissolve everything and collapse the building, providing antibiotic access," Berk said. "These can be targets for site-specific, antibiotic medicines in the future."
Super-resolution microscopy: painting with light
Berk is a biologist trained in physics and optics with expertise in imaging the structures of proteins: He was part of a team that a few years ago determined the atomic-scale structures of the ribosome, the cellular machine that translates genetic message into a finished protein.
He suspected that powerful new super-resolution light microscopy could reveal the unknown structure of biofilms. Super-resolution microscopy obtains 10 times better resolution than standard light microscopy -- 20 instead of 200 nanometers -- by highlighting only part of the image at a time using photo-switchable probes and compiling thousands of images into a single snapshot. The process is much like painting with light -- shining a flashlight beam on a dark scene while leaving the camera shutter open. Each snapshot may take a few minutes to compile, but for slow cellular growth, that's quick enough to obtain a stop-action movie.
The problem was how to label the cells with fluorescent dyes to continuously monitor their growth and division. Normally, biologists attach primary antibodies to cells, then flood the cells with fluorescent dye attached to a secondary antibody that latches onto the primary. They then flush away the excess dye, shine light on the dyed cells and photograph the fluorescence.
Berk suspected that a critically balanced concentration of fluorescent stain -- low enough to prevent background, but high enough to have efficient staining -- would work just as well and eliminate the need to flush out excess dye for fear it would create a background glow.
"The classical approach is first staining, then destaining, then taking only a single snapshot," Berk said. "We found a way to do staining and keep all the fluorescent probes inside the solution while we do the imaging, so we can continuously monitor everything, starting from a single cell all the way to a mature biofilm. Instead of one snapshot, we are recording a whole movie."
"It was a very simple, cool idea, but everyone thought it was crazy," he said. "Yes, it was crazy, but it worked."
Why are biofilms so hard to kill?
First there’s the slime, which antibiotics and chemicals have difficulty penetrating. In addition, electrical charges on the slime’s surface can form a barrier that keeps out antibiotics.
Because many cells deep within a biofilm are nutrient- and oxygen-starved, they grow fairly slowly — and are therefore less susceptible to antibiotics, which work best on actively dividing cells. To make matters worse, biofilms contain zombie-like “persister” cells which lie dormant when antibiotics are present but spring into action after antibiotic treatment ends.
Finally, cells within biofilms can organize themselves to pump drugs right out of cells — something Sauer called “a kind of bulimic behavior.”
Gene Regulation by Attached Cells
Evidence is mounting that up- and down-regulation of a number of genes occurs in the attaching cells upon initial interaction with the substratum. Davies and Geesey (34) demonstrated algC up-regulation in individual bacterial cells within minutes of attachment to surfaces in a flow cell system. This phenomenon is not limited to P. aeruginosa. Prigent-Combaret et al. (35) found that 22% of these genes were up-regulated in the biofilm state, and 16% were down-regulated. Becker et al. (36) showed that biofilms of Staphylococcus aureus were up-regulated for genes encoding enzymes involved in glycolysis or fermentation (phosphoglycerate mutase, triosephosphate isomerase, and alcohol dehydrogenase) and surmised that the up-regulation of these genes could be due to oxygen limitation in the developed biofilm, favoring fermentation. A recent study by Pulcini (37) also showed that algD, algU, rpoS, and genes controlling polyphosphokinase (PPK) synthesis were up-regulated in biofilm formation of P. aeruginosa. Prigent-Combaret et al. (35) opined that the expression of genes in biofilms is evidently modulated by the dynamic physicochemical factors external to the cell and may involve complex regulatory pathways.
Costerton JW (1999) Bacterial biofilms: a common cause of persistent infections. Science 284:1318–1322. https://doi.org/10.1126/science.284.5418.1318
O’Toole G, Kaplan HB, Kolter R (2000) Biofilm formation as microbial development. Annu Rev Microbiol 54(1):49–79. https://doi.org/10.1146/annurev.micro.54.1.49
Romling U, Kjelleberg S, Normark S, Nyman L, Uhlin BE, Akerlund B (2014) Microbial biofilm formation: a need to act. J Intern Med 276(2):98–110. https://doi.org/10.1111/joim.12242
Wolcott RD, Rhoads DD, Bennett ME, Wolcott BM, Gogokhia L, Costerton JW, Dowd SE (2010) Chronic wounds and the medical biofilm paradigm. J Wound Care 19(2):45–46, 8–50, 2–3. https://doi.org/10.12968/jowc.2010.19.2.46966
Soleimani N, Mobarez A, Olia M, Atyabi F (2015) Synthesis, characterization and effect of the antibacterial activity of chitosan nanoparticles on vancomycin-resistant Enterococcus and other gram negative or gram positive bacteria. Int J Pure Appl Sci Technol 26(1):14–23
Schembri MA, Kjærgaard K, Klemm P (2003) Global gene expression in Escherichia coli biofilms. Mol Microbiol 48(1):253–267. https://doi.org/10.1046/j.1365-2958.2003.03432.x
Thoendel M, Kavanaugh JS, Flack CE, Horswill AR (2011) Peptide signaling in the Staphylococci. Chem Rev 111:117–151. https://doi.org/10.1021/cr100370n
Robertson SR, McLean RJ (2015) Beneficial biofilms. AIMS Bioeng 2(4):437–448. https://doi.org/10.3934/bioeng.2015.4.437
Ramasamy M, Lee J (2016) Recent nanotechnology approaches for prevention and treatment of biofilm-associated infections on medical devices. Biomed Res Int 2016:1851242. https://doi.org/10.1155/2016/1851242
Donlan RM (2002) Biofilms: microbial life on surfaces. Emerg Infect Dis 8(9):881–890. https://doi.org/10.3201/eid0809.020063
Evans LV (2000) Biofilms: recent advances in their study and control. Harwood Academic, Amsterdam
Dunne WM (2002) Bacterial adhesion: Seen any good biofilms lately? Clin Microbiol Rev 15(2):155–166. https://doi.org/10.1128/CMR.15.2.155-166.2002
Cohen BE (2014) Functional linkage between genes that regulate osmotic stress responses and multidrug resistance transporters: challenges and opportunities for antibiotic discovery. Antimicrob Agents Chemother 58(2):640–646. https://doi.org/10.1128/AAC.02095-13
Rasamiravaka T, Labtani Q, Duez P, El Jaziri M (2015) The formation of biofilms by Pseudomonas aeruginosa : a review of the natural and synthetic compounds interfering with control mechanisms. Biomed Res Int 2015:1–17. https://doi.org/10.1155/2015/759348
Asally M et al (2012) Localized cell death focuses mechanical forces during 3D patterning in a biofilm. PNAS 109(46):18891–18896. https://doi.org/10.1073/pnas.1212429109
Rathsam C, Eaton RE, Simpson CL, Browne GV, Valova VA, Harty DWS, Jacques NA (2005) Two-dimensional fluorescence difference gel electrophoretic analysis of Streptococcus mutans biofilms. J Proteome Res 4:2161–2173
Islam N, Kim Y, Ross JM, Marten MR (2014) Proteome analysis of Staphylococcus aureus biofilm cells grown under physiologically relevant fluid shear conditions. Proteome Sci 12:21. https://doi.org/10.1186/1477-5956-12-21
Qayyum S, Sharma D, Bisht D, Khan AU (2016) Protein translation machinery holds a key for transition of planktonic cells to biofilm state in Enterococcus faecalis: a proteomic approach. Biochem Biophys Res Commun 474:652–659. https://doi.org/10.1016/j.bbrc.2016.04.145
Tielen P, Rosin N, Meyer AK, Dohnt K, Haddad I, Jänsch L, Klein J, Narten M, Pommerenke C, Scheer M, Schobert M, Schomburg D, Thielen B, Jahn D (2013) Regulatory and metabolic networks for the adaptation of Pseudomonas aeruginosa biofilms to urinary tract-like conditions. PLoS ONE 8(8):e71845. https://doi.org/10.1371/journal.pone.0071845
Otto M (2013) Staphylococcal infections: mechanisms of biofilm maturation and detachment as critical determinants of pathogenicity. Annu Rev Med 64:175–188. https://doi.org/10.1146/annurev-med-042711-140023
Annous BA, Fratamico PM, Smith JL (2009) Scientific status summary: quorum sensing in biofilms: Why bacteria behave the way they do? J Food Sci 74(1):R24–R37. https://doi.org/10.1111/j.1750-3841.2008.01022.x
Zhu J, Miller MB, Vance RE, Dziejman M, Bassler BL, Mekalanos JJ (2002) Quorum-sensing regulators control virulence gene expression in Vibrio cholerae. Proc Natl Acad Sci USA 99:3129–3134. https://doi.org/10.1073/pnas.052694299
Singh BN, Prateeksha UDK, Singh BR, Defoirdt T, Gupta VK, Vahabi K (2016) Bactericidal, quorum quenching and anti-biofilm nanofactories: a new niche for nanotechnologists. Crit Rev Biotechnol 37(4):525–540. https://doi.org/10.1080/07388551.2016.1199010
Lu TK, Collins JJ (2007) Dispersing biofilms with engineered enzymatic bacteriophage. PNAS 104:11197–11202. https://doi.org/10.1073/pnas.0704624104
Lewandowski Z, Evans LV (2000) Structure and function of biofilms: recent advances in their study and control. Harwood Academic Publishers, Amsterdam, pp 1–17
Bigger J (1944) Treatment of staphylococcal infections with penicillin-by intermittent sterilisation. Lancet 2:497–500
Fux CA, Costerton JW, Stewart PS, Stoodley P (2005) Survival strategies of infectious biofilms. Trends Microbiol 13:34–40. https://doi.org/10.1016/j.tim.2004.11.010
Vinodkumar C, Kalsurmath S, Neelagund Y (2008) Utility of lytic bacteriophage in the treatment of multidrug-resistant Pseudomonas aeruginosa septicemia in mice. Indian J Pathol Microbiol 51:360. https://doi.org/10.4103/0377-4929.42511
Waldrop R, McLaren A, Calara F, McLemore R (2014) Biofilm growth has a threshold response to glucose in vitro. Clin Orthop Relat Res 472(11):3305–3310. https://doi.org/10.1007/s11999-014-3538-5
Purevdorj B, Costerton JW, Stoodley P (2002) Influence of hydrodynamics and cell signaling on the structure and behavior of Pseudomonas aeruginosa biofilms. Appl Environ Microbiol 68(9):4457–4464
Sun J, Ziqing D, Aixin Y (2014) Bacterial multidrug efflux pumps: mechanisms, physiology and pharmacological exploitations. Biochem Biophys Res Commun 453(2):254–267. https://doi.org/10.1016/j.bbrc.2014.05.090
Wang L, Slayden RA, Barry CE III, Liu J (2000) Cell wall structure of a mutant of Mycobacterium smegmatis defective in the biosynthesis of mycolic acids. J Biol Chem 275:7224–7229
Neut D, Van Der Mei C, Bulstra HK, Busscher H (2007) The role of small-colony variants in failure to diagnose and treat biofilm infections in orthopedics. Acta Orthop Scand 78:299–308. https://doi.org/10.1080/17453670710013843
Høiby N, Frederiksen B, Pressler T (2005) Eradication of early Pseudomonas aeruginosa infection. J Cyst Fibros 4:49–54. https://doi.org/10.1016/j.jcf.2005.05.018
Daniel M, Chessman R, Al-Zahid S, Richards B, Rahman C, Ashraf W, McLaren J, Cox H, Qutachi O, Fortnum H, Fergie N, Shakesheff K, Birchall JP, Bayston RR (2012) Biofilm eradication with biodegradable modified-release antibiotic pellets: a potential treatment for glue ear. Arch Otolaryngol Head Neck Surg 138(10):942–949. https://doi.org/10.1001/archotol.2013.238
Gnanadhas DP, Elango M, Janardhanraj S et al (2015) Successful treatment of biofilm infections using shock waves combined with antibiotic therapy. Sci Rep 5:17440. https://doi.org/10.1038/srep17440
Singh PK, Schaefer AL, Parsek MR, Moninger TO, Welsh MJ, Greenberg EP (2000) Quorum-sensing signals indicate that cystic fibrosis lungs are infected with bacterial biofilms. Nature 407:762–764. https://doi.org/10.1038/35037627
Kokare CR, Chakraborty S, Khopade AN, Mahadik KR (2009) Biofilm: importance and applications. Indian J Biotechnol 8(2):159–168
Long B, Koyfman A (2018) Infectious endocarditis: an update for emergency clinicians. Am J Emerg Med 36(9):1686–1692. https://doi.org/10.1016/j.ajem.2018.06.074
Kokare CR, Kadam SS, Mahadik KR, Chopade BA (2007) Studies on bioemulsier production from marine Streptomyces sp. S1. Indian J Biotechnol 6(1):78–84
Overman PR (2007) Biofilm : a new view of plaque. J Contemp Dent Pract 1(3):18–29
Kumar V, Robbins SL (eds) (2007) Robbins basic pathology, 8th edn. Elsevier, Philadelphia
Alhede M, Alhede M (2014) The biofilm challenge. EWMA J 14:1–5
Gjødsbøl K, Christensen JJ, Karlsmark T, Jørgensen B, Klein BM, Krogfelt KA (2006) Multiple bacterial species reside in chronic wounds: a longitudinal study. Int Wound J 3:225–231. https://doi.org/10.1111/j.1742-481X.2006.00159.x
Bowling FL, Jude EB, Boulton AJM (2009) MRSA and diabetic foot wounds: contaminating or infecting organisms? Curr Diab Rep 9:440. https://doi.org/10.1007/s11892-009-0072-z
Bjarnsholt T (2013) The role of bacterial biofilms in chronic infections. APMIS 121:1–58. https://doi.org/10.1111/apm.12099
Foreman A, Wormald PJ (2010) Different biofilms, different disease? A clinical outcomes study. The Laryngoscope 120:1701–1706. https://doi.org/10.1002/lary.21024
Tambyah PA (2004) Catheter-associated urinary tract infections: diagnosis and prophylaxis. Int J Antimicrob Agents 24:44–48. https://doi.org/10.1016/j.ijantimicag.2004.02.008
Niveditha SN (2012) The isolation and the biofilm formation of uropathogens in the patients with catheter associated urinary tract infections (UTIs). J Clin Diagn Res. https://doi.org/10.7860/jcdr/2012/4367.2537
Jesaitis AJ, Franklin MJ, Berglund D, Sasaki M, Lord CI, Bleazard JB, Duffy JE, Beyenal H, Lewandowski Z (2003) Compromised host defence on Pseudomonas aeruginosa biofilms: characterization of neutrophil and biofilm interactions. J Immunol 171:4329–4339. https://doi.org/10.4049/jimmunol.171.8.4329
Bjarnsholt T, Jensen PØ, Fiandaca MJ, Pedersen J, Hansen CR, Andersen CB, Pressler T, Givskov M, Høiby N (2009) Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients. Pediatr Pulmonol 44:547–558. https://doi.org/10.1002/ppul.21011
Kolpen M et al (2009) Polymorphonuclear leukocytes consume oxygen in sputum from chronic Pseudomonas aeruginosa pneumonia in cystic fibrosis. Thorax. https://doi.org/10.1136/thx.2009.114512
McKeon DJ, Cadwallader KA, Idris S, Cowburn AS, Pasteur MC, Barker H, Haworth CS, Bilton D, Chilvers ER, Condliffe AM (2010) Cystic fibrosis neutrophils have normal intrinsic reactive oxygen species generation. Eur Respir J 35:1264–1272. https://doi.org/10.1183/09031936.00089709
Volk APD, Barber BM, Goss KL, Ruff JG, Heise CK, Hook JS, Moreland JG (2011) Priming of neutrophils and differentiated PLB-985 cells by pathophysiological concentrations of TNF-α: is partially oxygen dependent. J Innate Immun 3:298–314. https://doi.org/10.1159/000321439
Alhede M, Bjarnsholt T, Jensen PO, Phipps RK, Moser C, Christophersen L, Christensen LD, van Gennip M, Parsek M, Hoiby N, Rasmussen TB, Givskov M (2009) Pseudomonas aeruginosa recognizes and responds aggressively to the presence of polymorphonuclear leukocytes. Microbiology 155:3500–3508. https://doi.org/10.1099/mic.0.031443-0
Stewart PS, William Costerton J (2001) Antibiotic resistance of bacteria in biofilms. The Lancet 358:135–138. https://doi.org/10.1016/S0140-6736(01)05321-1
de Beer D, Stoodley P, Roe F, Lewandowski Z (1994) Effects of biofilm structures on oxygen distribution and mass transport. Biotechnol Bioeng 43:1131–1138. https://doi.org/10.1002/bit.260431118
Bjarnsholt T (2013) The role of bacterial biofilms in chronic infections. APMIS 121:1–58. https://doi.org/10.1111/apm.12099
Nadell CD, Xavier JB, Foster KR (2009) Thesociobiology of biofilms. FEMS Microbiol Rev 33:206–224. https://doi.org/10.1111/j.1574-6976.2008.00150.x
Camargo LFA, Marra AR, Büchele GL, Sogayar AMC, Cal RGR, de Sousa JMA, Silva E, Knobel E, Edmond MB (2009) Double-lumen central venous catheters impregnated with chlorhexidine and silver sulfadiazine to prevent catheter colonisation in the intensive care unit setting: a prospective randomised study. J Hosp Infect 72:227–233. https://doi.org/10.1016/j.jhin.2009.03.018
Bayston R, Fisher LE, Weber K (2009) An antimicrobial modified silicone peritoneal catheter with activity against both Gram positive and Gram negative bacteria. Biomaterials 30:3167–3173. https://doi.org/10.1016/j.biomaterials.2009.02.028
Bordi C, de Bentzmann S (2011) Hacking into bacterial biofilms: a new therapeutic challenge. Ann Intensive Care 1:19. https://doi.org/10.1186/2110-5820-1-19
Hasan J, Crawford RJ, Ivanova EP (2013) Antibacterial surfaces: the quest for a new generation of biomaterials. Trends Biotechnolt 31:295–304. https://doi.org/10.1016/j.tibtech.2013.01.017
Roosjen A, van der Mei HC, Busscher HJ, Norde W (2004) Microbial adhesion to poly(ethylene oxide) brushes: influence of polymer chain length and temperature. Langmuir 20:10949–10955. https://doi.org/10.1021/la048469l
Sousa C, Henriques M, Oliveira R (2011) Mini-review: antimicrobial central venous catheters–recent advances and strategies. Biofouling 27(6):609–620. https://doi.org/10.1080/08927014.2011.593261
Sun L, Zhang C, Li P (2012) Characterization, antibiofilm, and mechanism of action of novel PEG-stabilized lipid nanoparticles loaded with terpinen-4-ol. J Agric Food Chem 60:6150–6156. https://doi.org/10.1021/jf3010405
Webster T, Taylor J (2011) Reducing infections through nanotechnology and nanoparticles. Int J Nanomed. https://doi.org/10.2147/ijn.s22021
Suci PA, Berglund DL, Liepold L, Brumfield S, Pitts B, Davison W, Oltrogge L, Hoyt KO, Codd S, Stewart PS, Young M, Douglas T (2007) High-density targeting of a viral multifunctional nanoplatform to a pathogenic, biofilm-forming bacterium. Chem Biol 14:387–398. https://doi.org/10.1016/j.chembiol.2007.02.006
Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, Yacaman MJ (2005) The bactericidal effect of silver nanoparticles. Nanotechnology 16:2346
Pal S, Tak YK, Song JM (2007) Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli. Appl Environ Microbiol 73:1712–1720. https://doi.org/10.1128/AEM.02218-06
El Badawy AM, Silva RG, Morris B, Scheckel KG, Suidan MT, Tolaymat TM (2011) Surface charge-dependent toxicity of silver nanoparticles. Environ Sci Technol 45:283–287. https://doi.org/10.1021/es1034188
Lemire JA, Harrison JJ, Turner RJ (2013) Antimicrobial activity of metals: mechanisms, molecular targets and applications. Nat Rev Microbiol 11:371
Beyth N, Houri-Haddad Y, Domb A, Khan W, Hazan R (2015) Alternative antimicrobial approach: nano-antimicrobial materials. Evid Based Complement Altern Med 2015:1–16. https://doi.org/10.1155/2015/246012
Jones N, Ray B, Ranjit KT, Manna AC (2008) Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiol Lett 279:71–76. https://doi.org/10.1111/j.1574-6968.2007.01012.x
Baker C, Pradhan A, Pakstis L, Pochan D, Shah SI (2005) Synthesis and antibacterial properties of silver nanoparticles. JNN 5:244–249. https://doi.org/10.1166/jnn.2005.034
Ellis JR (2007) The many roles of silver in infection prevention. Am J Infect Control 35:E26. https://doi.org/10.1016/j.ajic.2007.04.017
Ansari M, Khan H, Khan A, Cameotra S, Alzohairy M (2015) Anti-biofilm efficacy of silver nanoparticles against MRSA and MRSE isolated from wounds in a tertiary care hospital. Indian J Med Microbiol 33:101. https://doi.org/10.4103/0255-0857.148402
Ahmed B, Hashmi A, Khan MS, Musarrat J (2018) ROS mediated destruction of cell membrane, growth and biofilms of human bacterial pathogens by stable metallic AgNPs functionalized from bell pepper extract and quercetin. Microb Pathog 111:375–387. https://doi.org/10.1016/j.micpath.2017.09.019
Ali K, Ahmed B, Dwivedi S, Saquib Q, Al-Khedhairy AA, Musarrat A (2015) Microwave accelerated green synthesis of stable silver nanoparticles with Eucalyptus globulus leaf extract and their antibacterial and antibiofilm activity on clinical isolates. J PLoS ONE 110(7):e0131178. https://doi.org/10.1371/journal.pone.0131178
Lee J-H, Kim Y-G, Cho MH, Lee J (2014) ZnO nanoparticles inhibit Pseudomonas aeruginosa biofilm formation and virulence factor production. Res Microbiol 169:888–896. https://doi.org/10.1016/j.micres.2014.05.005
Dhillon GS, Kaur S, Brar SK (2014) Facile fabrication and characterization of chitosan-based zinc oxide nanoparticles and evaluation of their antimicrobial and antibiofilm activity. Int Nano Lett. https://doi.org/10.1007/s40089-014-0107-6
Abdulkareem EH, Memarzadeh K, Allaker RP et al (2015) Anti-biofilm activity of zinc oxide and hydroxyapatite nanoparticles as dental implant coating materials. J Dent 43:1462–1469. https://doi.org/10.1016/j.jdent.2015.10.010
Applerot G, Lellouche J, Perkas N, Nitzan Y, Gedanken A, Banin E (2012) ZnO nanoparticle-coated surfaces inhibit bacterial biofilm formation and increase antibiotic susceptibility. RSC Adv 2:2314–2321
Al-Shabib NA, Husain FM, Hassan I et al (2018) Biofabrication of zinc oxide nanoparticle from Ochradenusbaccatus leaves: broad-spectrum antibiofilm activity, protein binding studies, and in vivo toxicity and stress studies. J Nanomater 2018:1–14. https://doi.org/10.1155/2018/8612158
Roudbar Mohammadi S, Mohammadi P, Hosseinkhani S, Shipour R (2013) Antifungal activity of TiO2 nanoparticles and EDTA on Candida albicans biofilms. Infect Epidemiol Med 1:33–38
Ohko Y, Nagao Y, Okano K, Sugiura N, Fukuda A, Yang Y, Negishi N, Takeuchi M, Hanada S (2009) Prevention of Phormidium tenue biofilm formation by TiO2 photocatalysis. Microbes Environ 24:241–245. https://doi.org/10.1264/jsme2.ME09106
Khan ST, Ahmad J, Ahamed M et al (2016) Zinc oxide and titanium dioxide nanoparticles induce oxidative stress, inhibit growth, and attenuate biofilm formation activity of Streptococcus mitis. JBIC 21:295–303. https://doi.org/10.1007/s00775-016-1339-x
Ren G, Hu D, Cheng EWC, Vargas-Reus MA, Reip P, Allaker RP (2009) Characterisation of copper oxide nanoparticles for antimicrobial applications. Int J Antimicrob Agents 33:587–590. https://doi.org/10.1016/j.ijantimicag.2008.12.004
Eshed M, Lellouche J, Matalon S, Gedanken A, Banin E (2012) Sonochemical coatings of ZnO and CuO nanoparticles inhibit Streptococcus mutans biofilm formation on teeth model. Langmuir 28:12288–12295. https://doi.org/10.1021/la301432a
LewisOscar F, MubarakAli D, Nithya C et al (2015) One pot synthesis and anti-biofilm potential of copper nanoparticles (CuNPs) against clinical strains of Pseudomonas aeruginosa. Biofouling 31:379–391. https://doi.org/10.1080/08927014.2015.1048686
Agarwala M, Choudhury B, Yadav RNS (2014) Comparative study of antibiofilm activity of copper oxide and iron oxide nanoparticles against multidrug resistant biofilm forming uropathogens. Indian J Microbiol 54:365–368. https://doi.org/10.1007/s12088-014-0462-z
Eshed M, Lellouche J, Gedanken A, Banin E (2014) A Zn-doped CuO nanocomposite shows enhanced antibiofilm and antibacterial activities against Streptococcus mutans compared to nanosized CuO. Adv Funct Mater 24:1382–1390. https://doi.org/10.1002/adfm.201302425
Singh A, Ahmed A, Prasad KN, Khanduja S, Singh SK, Srivastava JK, Gajbhiye NS (2015) Antibiofilm and membrane-damaging potential of cuprous oxide nanoparticles against Staphylococcus aureus with reduced susceptibility to vancomycin. Antimicrob Agents Chemother 59:6882–6890. https://doi.org/10.1128/AAC.01440-15
Yu Q, Li J, Zhang Y, Wang Y, Liu L, Li M (2016) Inhibition of gold nanoparticles (AuNPs) on pathogenic biofilm formation and invasion to host cells. Sci Rep 6:26667
Chen W-Y, Lin J-Y, Chen W-J, Luo L, Wei-Guang Diau E, Chen Y-C (2010) Functional gold nanoclusters as antimicrobial agents for antibiotic-resistant bacteria. Nanomedicine 5:755–764. https://doi.org/10.2217/nnm.10.43
deAlteriis E, Maselli V, Falanga A et al (2018) Efficiency of gold nanoparticles coated with the antimicrobial peptide indolicidin against biofilm formation and development of Candida spp. clinical isolates. Infect Drug Resist 11:915–925. https://doi.org/10.2147/IDR.S164262
Vinoj G, Pati R, Sonawane A, Vaseeharan B (2015) In vitro cytotoxic effects of gold nanoparticles coated with functional acyl homoserine lactone lactonase protein from Bacillus licheniformis and their antibiofilm activity against Proteus species. Antimicrob Agents Chemother 59:763–771. https://doi.org/10.1128/AAC.03047-14
Manju S, Malaikozhundan B, Vijayakumar S, Shanthi S, Jaishabanu A, Ekambaram P, Vaseeharan B (2016) Antibacterial, antibiofilm and cytotoxic effects of Nigella sativa essential oil coated gold nanoparticles. Microb Pathog 91:129–135. https://doi.org/10.1016/j.micpath.2015.11.021
Gopinath K, Kumaraguru S, Bhakyaraj K, Mohan S, Venkatesh KS, Esakkirajan M, Kaleeswarran P, Alharbi NS, Kadaikunnan S, Govindarajan M, Benelli G, Arumugam A (2016) Green synthesis of silver, gold and silver/gold bimetallic nanoparticles using the Gloriosa superba leaf extract and their antibacterial and antibiofilm activities. Microb Pathog 101:1–11. https://doi.org/10.1016/j.micpath.2016.10.011
Haghighi F, Mohammadi SR, Mohammadi P, Hosseinkhani S, Shidpour R (2013) Antifungal Activity of TiO2 nanoparticles and EDTA on Candida albicans Biofilms. Infect Epidemiol Med 1:33–38
Kang S, Mauter MS, Elimelech M (2009) Microbial cytotoxicity of carbon-based nanomaterials: implications for river water and wastewater effluent. Environ Sci Technol 43:2648–2653. https://doi.org/10.1021/es8031506
Lichter JA, Rubner MF (2009) Polyelectrolyte multilayers with intrinsic antimicrobial functionality: the importance of mobile polycations. Langmuir 25:7686–7694. https://doi.org/10.1021/la900349c
Nevius BA, Chen YP, Ferry JL, Decho AW (2012) Surface-functionalization effects on uptake of fluorescent polystyrene nanoparticles by model biofilms. Ecotoxicology 21:2205–2213. https://doi.org/10.1007/s10646-012-0975-3
Lee ALZ, Ng VWL, Wang W, Hedrick JL, Yang YY (2013) Block copolymer mixtures as antimicrobial hydrogels for biofilm eradication. Biomaterials 34:10278–10286. https://doi.org/10.1016/j.biomaterials.2013.09.029
Tamilvanan S, Venkateshan N, Ludwig A (2008) The potential of lipid- and polymer-based drug delivery carriers for eradicating biofilm consortia on device-related nosocomial infections. J Control Release 128:2–22. https://doi.org/10.1016/j.jconrel.2008.01.006
DiTizio V, Ferguson GW, Mittelman MW, Khoury AE, Bruce AW, Di Cosmo F (1998) A liposomal hydrogel for the prevention of bacterial adhesion to catheters. Biomaterials 19:1877–1884. https://doi.org/10.1016/S0142-9612(98)00096-9
Al-Adham ISI, Al-Hmoud ND, Khalil E, Kierans M, Collier PJ (2003) Microemulsions are highly effective anti-biofilm agents. Lett Appl Microbiol 36:97–100. https://doi.org/10.1046/j.1472-765X.2003.01266.x
Al-Adham ISI, Ashour H, Al-Kaissi E, Khalil E, Kierans M, Collier PJ (2013) Studies on the kinetics of killing and the proposed mechanism of action of microemulsions against fungi. Int J Pharm 454:226–232. https://doi.org/10.1016/j.ijpharm.2013.06.049
Ramalingam K, Frohlich NC, Lee VA (2013) Effect of nanoemulsion on dental unit waterline biofilm. J Dent 8:333–336. https://doi.org/10.1016/j.jds.2013.02.035
Janiszewska J, Swieton J, Lipkowski AW, Urbanczyk-Lipkowska Z (2003) Low molecular mass peptide dendrimers that express antimicrobial properties. Bioorg Med Chem Lett 13:3711–3713. https://doi.org/10.1016/j.bmcl.2003.08.009
Johansson EMV, Crusz SA, Kolomiets E, Buts L, Kadam RU, Cacciarini M, Bartels K-M, Diggle SP, Cámara M, Williams P, Loris R, Nativi C, Rosenau F, Jaeger K-E, Darbre T, Reymond J-L (2008) Inhibition and dispersion of Pseudomonas aeruginosa biofilms by glycopeptide dendrimers targeting the fucose-specific lectin LecB. Chem Biol 15:1249–1257. https://doi.org/10.1016/j.chembiol.2008.10.009
Lucky SS, Soo KC, Zhang Y (2015) Nanoparticles in photodynamic therapy. Chem Rev 115:1990–2042. https://doi.org/10.1021/cr5004198
The Role of Bacterial Biofilms in Ocular Infections
There is increasing evidence that bacterial biofilms play a role in a variety of ocular infections. Bacterial growth is characterized as a biofilm when bacteria attach to a surface and/or to each other. This is distinguished from a planktonic or free-living mode of bacterial growth where these interactions are not present. Biofilm formation is a genetically controlled process in the life cycle of bacteria resulting in numerous changes in the cellular physiology of the organism, often including increased antibiotic resistance compared to growth under planktonic conditions. The presence of bacterial biofilms has been demonstrated on many medical devices including intravenous catheters, as well as materials relevant to the eye such as contact lenses, scleral buckles, suture material, and intraocular lenses. Many ocular infections often occur when such prosthetic devices come in contact with or are implanted in the eye. For instance, 56% of corneal ulcers in the United States are associated with contact lens wear. Bacterial biofilms may participate in ocular infections by allowing bacteria to persist on abiotic surfaces that come in contact with, or are implanted in the eye, and by direct biofilm formation on the biotic surfaces of the eye. An understanding of the role of bacterial biofilm formation in ocular infections may aid in the development of future antimicrobial strategies in ophthalmology. We review the current literature and concepts relating to biofilm formation and infections of the eye.
Biofilm formation begins when free-floating microorganisms such as bacteria come in contact with an appropriate surface and begin to put down roots, so to speak. This first step of attachment occurs when the microorganisms produce a gooey substance known as an extracellular polymeric substance (EPS), according to the Center for Biofilm Engineering at Montana State University. An EPS is a network of sugars, proteins and nucleic acids (such as DNA). It enables the microorganisms in a biofilm to stick together.
Attachment is followed by a period of growth. Further layers of microorganisms and EPS build upon the first layers. Ultimately, they create a bulbous and complex 3D structure, according to the Center for Biofilm Engineering. Water channels crisscross biofilms and allow for the exchange of nutrients and waste products, according to the article in Microbe.
Multiple environmental conditions help determine the extent to which a biofilm grows. These factors also determine whether it is made of only a few layers of cells or significantly more. "It really depends on the biofilm," said Robin Gerlach, a professor in the department of chemical and biological engineering at Montana State University-Bozeman. For instance, microorganisms that produce a large amount of EPS can grow into fairly thick biofilms even if they do not have access to a lot of nutrients, he said. On the other hand, for microorganisms that depend on oxygen, the amount available can limit how much they can grow. Another environmental factor is the concept of "shear stress." "If you have a very high flow [of water] across a biofilm, like in a creek, the biofilm is usually fairly thin. If you have a biofilm in slow flowing water, like in a pond, it can become very thick," Gerlach explained.
Finally, the cells within a biofilm can leave the fold and establish themselves on a new surface. Either a clump of cells breaks away, or individual cells burst out of the biofilm and seek out a new home. This latter process is known as "seeding dispersal," according to the Center for Biofilm Engineering.
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Financial & competing interests disclosure
This work was supported by a grant from the National Institutes of Health, National Institute of Allergy and Infectious Disease P01 AI083211. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.