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13.5: Fungi - Biology

13.5: Fungi - Biology


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The word fungus comes from the Latin word for mushroom. Edible mushrooms, yeasts, black mold, and Penicillium notatum (the producer of the antibiotic penicillin) are all members of the kingdom Fungi, which belongs to the domain Eukarya. As eukaryotes, a typical fungal cell contains a true nucleus and many membrane-bound organelles.

Fungi were once considered plant-like organisms; however, DNA comparisons have shown that fungi are more closely related to animals than plants. Fungi are not capable of photosynthesis: They use complex organic compounds as sources of energy and carbon. Some fungal organisms multiply only asexually, whereas others undergo both asexual reproduction and sexual reproduction. Most fungi produce a large number of spores that are disseminated by the wind. Like bacteria, fungi play an essential role in ecosystems, because they are decomposers and participate in the cycling of nutrients by breaking down organic materials into simple molecules.

Fungi often interact with other organisms, forming mutually beneficial or mutualistic associations. Fungi also cause serious infections in plants and animals. For example, Dutch elm disease is a particularly devastating fungal infection that destroys many native species of elm (Ulmus spp.). The fungus infects the vascular system of the tree. It was accidentally introduced to North America in the 1900s and decimated elm trees across the continent. Dutch elm disease is caused by the fungus Ophiostoma ulmi. The elm bark beetle acts as a vector and transmits the disease from tree to tree. Many European and Asiatic elms are less susceptible than American elms.

In humans, fungal infections are generally considered challenging to treat because, unlike bacteria, they do not respond to traditional antibiotic therapy since they are also eukaryotes. These infections may prove deadly for individuals with a compromised immune system.

Fungi have many commercial applications. The food industry uses yeasts in baking, brewing, and wine making. Many industrial compounds are byproducts of fungal fermentation. Fungi are the source of many commercial enzymes and antibiotics.

Cell Structure and Function

Fungi are eukaryotes and as such have a complex cellular organization. As eukaryotes, fungal cells contain a membrane-bound nucleus. A few types of fungi have structures comparable to the plasmids (loops of DNA) seen in bacteria. Fungal cells also contain mitochondria and a complex system of internal membranes, including the endoplasmic reticulum and Golgi apparatus.

Fungal cells do not have chloroplasts. Although the photosynthetic pigment chlorophyll is absent, many fungi display bright colors, ranging from red to green to black. The poisonous Amanita muscaria (fly agaric) is recognizable by its bright red cap with white patches (Figure 13.4.2). Pigments in fungi are associated with the cell wall and play a protective role against ultraviolet radiation. Some pigments are toxic.

Like plant cells, fungal cells are surrounded by a thick cell wall; however, the rigid layers contain the complex polysaccharides chitin and glucan and not cellulose that is used by plants. Chitin, also found in the exoskeleton of insects, gives structural strength to the cell walls of fungi. The cell wall protects the cell from desiccation and predators. Fungi have plasma membranes similar to other eukaryotes, except that the structure is stabilized by ergosterol, a steroid molecule that functions like the cholesterol found in animal cell membranes. Most members of the kingdom Fungi are nonmotile. Flagella are produced only by the gametes in the primitive division Chytridiomycota.

Growth and Reproduction

The vegetative body of a fungus is called a thallus and can be unicellular or multicellular. Some fungi are dimorphic because they can go from being unicellular to multicellular depending on environmental conditions. Unicellular fungi are generally referred to as yeasts.Saccharomyces cerevisiae (baker’s yeast) and Candida species (the agents of thrush, a common fungal infection) are examples of unicellular fungi.

Most fungi are multicellular organisms. They display two distinct morphological stages: vegetative and reproductive. The vegetative stage is characterized by a tangle of slender thread-like structures called hyphae (singular, hypha), whereas the reproductive stage can be more conspicuous. A mass of hyphae is called a mycelium (Figure 13.4.3). It can grow on a surface, in soil or decaying material, in a liquid, or even in or on living tissue. Although individual hypha must be observed under a microscope, the mycelium of a fungus can be very large with some species truly being “the fungus humongous.” The giant Armillaria ostoyae (honey mushroom) is considered the largest organism on Earth, spreading across over 2,000 acres of underground soil in eastern Oregon; it is estimated to be at least 2,400 years old.

Most fungal hyphae are divided into separate cells by end walls called septa (singular, septum). In most divisions (like plants, fungal phyla are called divisions by tradition) of fungi, tiny holes in the septa allow for the rapid flow of nutrients and small molecules from cell to cell along the hyphae. They are described as perforated septa. The hyphae in bread molds (which belong to the division Zygomycota) are not separated by septa. They are formed of large cells containing many nuclei, an arrangement described as coenocytic hyphae.

Fungi thrive in environments that are moist and slightly acidic, and can grow with or without light. They vary in their oxygen requirements. Most fungi are obligate aerobes, requiring oxygen to survive. Other species, such as the Chytridiomycota that reside in the rumen of cattle, are obligate anaerobes, meaning that they cannot grow and reproduce in an environment with oxygen. Yeasts are intermediate: They grow best in the presence of oxygen but can use fermentation in the absence of oxygen. The alcohol produced from yeast fermentation is used in wine and beer production, and the carbon dioxide they produce carbonates beer and sparkling wine, and makes bread rise.

Fungi can reproduce sexually or asexually. In both sexual and asexual reproduction, fungi produce spores that disperse from the parent organism by either floating in the wind or hitching a ride on an animal. Fungal spores are smaller and lighter than plant seeds, but they are not usually released as high in the air. The giant puffball mushroom bursts open and releases trillions of spores: The huge number of spores released increases the likelihood of spores landing in an environment that will support growth (Figure 13.4.4).

How Fungi Obtain Nutrition

Like animals, fungi are heterotrophs: They use complex organic compounds as a source of carbon rather than fixing carbon dioxide from the atmosphere, as some bacteria and most plants do. In addition, fungi do not fix nitrogen from the atmosphere. Like animals, they must obtain it from their diet. However, unlike most animals that ingest food and then digest it internally in specialized organs, fungi perform these steps in the reverse order. Digestion precedes ingestion. First, exoenzymes, enzymes that catalyze reactions on compounds outside of the cell, are transported out of the hyphae where they break down nutrients in the environment. Then, the smaller molecules produced by the external digestion are absorbed through the large surface areas of the mycelium. As with animal cells, the fungal storage polysaccharide is glycogen rather than starch, as found in plants.

Fungi are mostly saprobes, organisms that derive nutrients from decaying organic matter. They obtain their nutrients from dead or decomposing organic matter, mainly plant material. Fungal exoenzymes are able to break down insoluble polysaccharides, such as the cellulose and lignin of dead wood, into readily absorbable glucose molecules. Decomposers are important components of ecosystems, because they return nutrients locked in dead bodies to a form that is usable for other organisms. This role is discussed in more detail later. Because of their varied metabolic pathways, fungi fulfill an important ecological role and are being investigated as potential tools in bioremediation. For example, some species of fungi can be used to break down diesel oil and polycyclic aromatic hydrocarbons. Other species take up heavy metals such as cadmium and lead.

Fungal Diversity

The kingdom Fungi contains four major divisions that were established according to their mode of sexual reproduction. Polyphyletic, unrelated fungi that reproduce without a sexual cycle, are placed for convenience in a fifth division, and a sixth major fungal group that does not fit well with any of the previous five has recently been described. Not all mycologists agree with this scheme. Rapid advances in molecular biology and the sequencing of 18S rRNA (a component of ribosomes) continue to reveal new and different relationships between the various categories of fungi.

The traditional divisions of Fungi are the Chytridiomycota (chytrids), the Zygomycota(conjugated fungi), the Ascomycota (sac fungi), and the Basidiomycota (club fungi). An older classification scheme grouped fungi that strictly use asexual reproduction into Deuteromycota, a group that is no longer in use. The Glomeromycota belong to a newly described group (Figure 13.4.5).

Pathogenic Fungi

Many fungi have negative impacts on other species, including humans and the organisms they depend on for food. Fungi may be parasites, pathogens, and, in a very few cases, predators.

Plant Parasites and Pathogens

The production of enough good-quality crops is essential to our existence. Plant diseases have ruined crops, bringing widespread famine. Most plant pathogens are fungi that cause tissue decay and eventual death of the host (Figure 13.4.6). In addition to destroying plant tissue directly, some plant pathogens spoil crops by producing potent toxins. Fungi are also responsible for food spoilage and the rotting of stored crops. For example, the fungus Claviceps purpurea causes ergot, a disease of cereal crops (especially of rye). Although the fungus reduces the yield of cereals, the effects of the ergot’s alkaloid toxins on humans and animals are of much greater significance: In animals, the disease is referred to as ergotism. The most common signs and symptoms are convulsions, hallucination, gangrene, and loss of milk in cattle. The active ingredient of ergot is lysergic acid, which is a precursor of the drug LSD. Smuts, rusts, and powdery or downy mildew are other examples of common fungal pathogens that affect crops.


Figure 13.4.6: Some fungal pathogens include (a) green mold on grapefruit, (b) fungus on grapes, (c) powdery mildew on a zinnia, and (d) stem rust on a sheaf of barley. Notice the brownish color of the fungus in (b) Botrytis cinerea, also referred to as the “noble rot,” which grows on grapes and other fruit. Controlled infection of grapes by Botrytis is used to produce strong and much-prized dessert wines. (credit a: modification of work by Scott Bauer, USDA ARS; credit b: modification of work by Stephen Ausmus, USDA ARS; credit c: modification of work by David Marshall, USDA ARS; credit d: modification of work by Joseph Smilanick, USDA ARS)

Aflatoxins are toxic and carcinogenic compounds released by fungi of the genus Aspergillus. Periodically, harvests of nuts and grains are tainted by aflatoxins, leading to massive recall of produce, sometimes ruining producers, and causing food shortages in developing countries.

Animal and Human Parasites and Pathogens

Fungi can affect animals, including humans, in several ways. Fungi attack animals directly by colonizing and destroying tissues. Humans and other animals can be poisoned by eating toxic mushrooms or foods contaminated by fungi. In addition, individuals who display hypersensitivity to molds and spores develop strong and dangerous allergic reactions. Fungal infections are generally very difficult to treat because, unlike bacteria, fungi are eukaryotes. Antibiotics only target prokaryotic cells, whereas compounds that kill fungi also adversely affect the eukaryotic animal host.

Many fungal infections (mycoses) are superficial and termed cutaneous (meaning “skin”) mycoses. They are usually visible on the skin of the animal. Fungi that cause the superficial mycoses of the epidermis, hair, and nails rarely spread to the underlying tissue (Figure 13.4.7). These fungi are often misnamed “dermatophytes” from the Greek dermis skin and phyte plant, but they are not plants. Dermatophytes are also called “ringworms” because of the red ring that they cause on skin (although the ring is caused by fungi, not a worm). These fungi secrete extracellular enzymes that break down keratin (a protein found in hair, skin, and nails), causing a number of conditions such as athlete’s foot, jock itch, and other cutaneous fungal infections. These conditions are usually treated with over-the-counter topical creams and powders, and are easily cleared. More persistent, superficial mycoses may require prescription oral medications.

Systemic mycoses spread to internal organs, most commonly entering the body through the respiratory system. For example, coccidioidomycosis (valley fever) is commonly found in the southwestern United States, where the fungus resides in the dust. Once inhaled, the spores develop in the lungs and cause signs and symptoms similar to those of tuberculosis. Histoplasmosis (Figure 13.4.7c) is caused by the dimorphic fungus Histoplasma capsulatum; it causes pulmonary infections and, in rare cases, swelling of the membranes of the brain and spinal cord. Treatment of many fungal diseases requires the use of antifungal medications that have serious side effects.

Opportunistic mycoses are fungal infections that are either common in all environments or part of the normal biota. They affect mainly individuals who have a compromised immune system. Patients in the late stages of AIDS suffer from opportunistic mycoses, such as Pneumocystis, which can be life threatening. The yeast Candida spp., which is a common member of the natural biota, can grow unchecked if the pH, the immune defenses, or the normal population of bacteria is altered, causing yeast infections of the vagina or mouth (oral thrush).

Fungi may even take on a predatory lifestyle. In soil environments that are poor in nitrogen, some fungi resort to predation of nematodes (small roundworms). Species of Arthrobotrys fungi have a number of mechanisms to trap nematodes. For example, they have constricting rings within their network of hyphae. The rings swell when the nematode touches it and closes around the body of the nematode, thus trapping it. The fungus extends specialized hyphae that can penetrate the body of the worm and slowly digest the hapless prey.

Beneficial Fungi

Fungi play a crucial role in the balance of ecosystems. They colonize most habitats on Earth, preferring dark, moist conditions. They can thrive in seemingly hostile environments, such as the tundra, thanks to a most successful symbiosis with photosynthetic organisms, like lichens. Fungi are not obvious in the way that large animals or tall trees are. Yet, like bacteria, they are major decomposers of nature. With their versatile metabolism, fungi break down organic matter that is insoluble and would not be recycled otherwise.

Importance to Ecosystems

Food webs would be incomplete without organisms that decompose organic matter and fungi are key participants in this process. Decomposition allows for cycling of nutrients such as carbon, nitrogen, and phosphorus back into the environment so they are available to living things, rather than being trapped in dead organisms. Fungi are particularly important because they have evolved enzymes to break down cellulose and lignin, components of plant cell walls that few other organisms are able to digest, releasing their carbon content.

Fungi are also involved in ecologically important coevolved symbioses, both mutually beneficial and pathogenic with organisms from the other kingdoms. Mycorrhiza, a term combining the Greek roots myco meaning fungus and rhizo meaning root, refers to the association between vascular plant roots and their symbiotic fungi. Somewhere between 80–90 percent of all plant species have mycorrhizal partners. In a mycorrhizal association, the fungal mycelia use their extensive network of hyphae and large surface area in contact with the soil to channel water and minerals from the soil into the plant. In exchange, the plant supplies the products of photosynthesis to fuel the metabolism of the fungus. Ectomycorrhizae (“outside” mycorrhiza) depend on fungi enveloping the roots in a sheath (called a mantle) and a net of hyphae that extends into the roots between cells. In a second type, the Glomeromycota fungi form arbuscular mycorrhiza. In these mycorrhiza, the fungi form arbuscles, a specialized highly branched hypha, which penetrate root cells and are the sites of the metabolic exchanges between the fungus and the host plant. Orchids rely on a third type of mycorrhiza. Orchids form small seeds without much storage to sustain germination and growth. Their seeds will not germinate without a mycorrhizal partner (usually Basidiomycota). After nutrients in the seed are depleted, fungal symbionts support the growth of the orchid by providing necessary carbohydrates and minerals. Some orchids continue to be mycorrhizal throughout their lifecycle.

Lichens blanket many rocks and tree bark, displaying a range of colors and textures. Lichens are important pioneer organisms that colonize rock surfaces in otherwise lifeless environments such as are created by glacial recession. The lichen is able to leach nutrients from the rocks and break them down in the first step to creating soil. Lichens are also present in mature habitats on rock surfaces or the trunks of trees. They are an important food source for caribou. Lichens are not a single organism, but rather a fungus (usually an Ascomycota or Basidiomycota species) living in close contact with a photosynthetic organism (an alga or cyanobacterium). The body of a lichen, referred to as a thallus, is formed of hyphae wrapped around the green partner. The photosynthetic organism provides carbon and energy in the form of carbohydrates and receives protection from the elements by the thallus of the fungal partner. Some cyanobacteria fix nitrogen from the atmosphere, contributing nitrogenous compounds to the association. In return, the fungus supplies minerals and protection from dryness and excessive light by encasing the algae in its mycelium. The fungus also attaches the symbiotic organism to the substrate.

Fungi have evolved mutualistic associations with numerous arthropods. The association between species of Basidiomycota and scale insects is one example. The fungal mycelium covers and protects the insect colonies. The scale insects foster a flow of nutrients from the parasitized plant to the fungus. In a second example, leaf-cutting ants of Central and South America literally farm fungi. They cut disks of leaves from plants and pile them up in gardens. Fungi are cultivated in these gardens, digesting the cellulose that the ants cannot break down. Once smaller sugar molecules are produced and consumed by the fungi, they in turn become a meal for the ants. The insects also patrol their garden, preying on competing fungi. Both ants and fungi benefit from the association. The fungus receives a steady supply of leaves and freedom from competition, while the ants feed on the fungi they cultivate.

Importance to Humans

Although we often think of fungi as organisms that cause diseases and rot food, fungi are important to human life on many levels. As we have seen, they influence the well-being of human populations on a large scale because they help nutrients cycle in ecosystems. They have other ecosystem roles as well. For example, as animal pathogens, fungi help to control the population of damaging pests. These fungi are very specific to the insects they attack and do not infect other animals or plants. The potential to use fungi as microbial insecticides is being investigated, with several species already on the market. For example, the fungus Beauveria bassiana is a pesticide that is currently being tested as a possible biological control for the recent spread of emerald ash borer. It has been released in Michigan, Illinois, Indiana, Ohio, West Virginia, and Maryland.

The mycorrhizal relationship between fungi and plant roots is essential for the productivity of farmland. Without the fungal partner in the root systems, 80–90% of trees and grasses would not survive. Mycorrhizal fungal inoculants are available as soil amendments from gardening supply stores and promoted by supporters of organic agriculture.

We also eat some types of fungi. Mushrooms figure prominently in the human diet. Morels, shiitake mushrooms, chanterelles, and truffles are considered delicacies (Figure 13.4.8). The humble meadow mushroom, Agaricus campestris, appears in many dishes. Molds of the genus Penicillium ripen many cheeses. They originate in the natural environment such as the caves of Roquefort, France, where wheels of sheep milk cheese are stacked to capture the molds responsible for the blue veins and pungent taste of the cheese.

Fermentation—of grains to produce beer, and of fruits to produce wine—is an ancient art that humans in most cultures have practiced for millennia. Wild yeasts are acquired from the environment and used to ferment sugars into CO2 and ethyl alcohol under anaerobic conditions. It is now possible to purchase isolated strains of wild yeasts from different wine-making regions. Pasteur was instrumental in developing a reliable strain of brewer’s yeast, Saccharomyces cerevisiae, for the French brewing industry in the late 1850s. It was one of the first examples of biotechnology patenting. Yeast is also used to make breads that rise. The carbon dioxide they produce is responsible for the bubbles produced in the dough that become the air pockets of the baked bread.

Many secondary metabolites of fungi are of great commercial importance. Antibiotics are naturally produced by fungi to kill or inhibit the growth of bacteria, and limit competition in the natural environment. Valuable drugs isolated from fungi include the immunosuppressant drug cyclosporine (which reduces the risk of rejection after organ transplant), the precursors of steroid hormones, and ergot alkaloids used to stop bleeding. In addition, as easily cultured eukaryotic organisms, some fungi are important model research organisms including the red bread mold Neurospora crassa and the yeast, S. cerevisiae.

Section Summary

Fungi are eukaryotic organisms that appeared on land over 450 million years ago. They are heterotrophs and contain neither photosynthetic pigments such as chlorophylls nor organelles such as chloroplasts. Because they feed on decaying and dead matter, they are saprobes. Fungi are important decomposers and release essential elements into the environment. External enzymes digest nutrients that are absorbed by the body of the fungus called a thallus. A thick cell wall made of chitin surrounds the cell. Fungi can be unicellular as yeasts or develop a network of filaments called a mycelium, often described as mold. Most species multiply by asexual and sexual reproductive cycles, and display an alternation of generations.

The divisions of fungi are the Chytridiomycota, Zygomycota, Ascomycota, Basidiomycota, and Glomeromycota.

Fungi establish parasitic relationships with plants and animals. Fungal diseases can decimate crops and spoil food during storage. Compounds produced by fungi can be toxic to humans and other animals. Mycoses are infections caused by fungi. Superficial mycoses affect the skin, whereas systemic mycoses spread through the body. Fungal infections are difficult to cure.

Fungi have colonized all environments on Earth but are most often found in cool, dark, moist places with a supply of decaying material. Fungi are important decomposers because they are saprobes. Many successful mutualistic relationships involve a fungus and another organism. They establish complex mycorrhizal associations with the roots of plants. Lichens are a symbiotic relationship between a fungus and a photosynthetic organism, usually an alga or cyanobacterium.

Fungi are important to everyday human life. Fungi are important decomposers in most ecosystems. Mycorrhizal fungi are essential for the growth of most plants. Fungi, as food, play a role in human nutrition in the form of mushrooms and as agents of fermentation in the production of bread, cheeses, alcoholic beverages, and numerous other food preparations. Secondary metabolites of fungi are used in medicine as antibiotics and anticoagulants. Fungi are used in research as model organisms for the study of eukaryotic genetics and metabolism.

Multiple Choice

Which polysaccharide is usually found in the cell walls of fungi?

A. starch
B. glycogen
C. chitin
D. cellulose

C

What term describes the close association of a fungus with the root of a tree?

A. a rhizoid
B. a lichen
C. a mycorrhiza
D. an endophyte

C

Free Response

Why can superficial mycoses in humans lead to bacterial infections?

Dermatophytes that colonize skin break down the keratinized layer of dead cells that protects tissues from bacterial invasion. Once the integrity of the skin is breached, bacteria can enter the deeper layers of tissues and cause infections.

Glossary

Ascomycota
(sac fungi) a division of fungi that store spores in a sac called ascus
basidiomycota
(club fungi) a division of fungi that produce club shaped structures, basidia, which contain spores
Chytridiomycota
(chytrids) a primitive division of fungi that live in water and produce gametes with flagella
Glomeromycota
a group of fungi that form symbiotic relationships with the roots of trees
hypha
a fungal filament composed of one or more cells
lichen
the close association of a fungus with a photosynthetic alga or bacterium that benefits both partners
mold
a tangle of visible mycelia with a fuzzy appearance
mycelium
a mass of fungal hyphae
mycorrhiza
a mutualistic association between fungi and vascular plant roots
mycosis
a fungal infection
septum
the cell wall division between hyphae
thallus
a vegetative body of a fungus
yeast
a general term used to describe unicellular fungi

13.5: Fungi - Biology

In recent years the development of new molecular biology tools and the elucidation of whole genome sequences have revolutionized research on pathogenic fungi. Such advances have led to the development of faster, more reliable diagnostic techniques for medically important pathogens such as Candida albicans, Aspergillus fumigatus and Cryptococcus neoformans. In addition they have led to a major breakthrough in the approach for the generation of novel anti-fungal agents. Now it is possible to search for agents that target essential genes. Research in this area has never been more exciting.

In this book a panel of expert international mycologists critically review the most important cutting-edge topics. Chapters are written from a molecular and genomic perspective and through the provision of extensive reference sections positively encourage readers to pursue the subject in greater detail. Topics include: gene expression and regulation, heterozygosity in Candida, molecular diagnosis, regulation of the host-fungal interaction, the development of anti-fungals, signal transduction, and mechanisms of multi-drug resistance. Essential reading for everyone with an interest in pathogenic fungi including: mycologists, biotechnologists, molecular biologists, and pharmaceutical and biotechnology companies.

". this book provides a timely survey of key topics . The book clearly meets the stated aim of the editors '. to help the busy research scientist and/or teacher of medical mycology to keep abreast of all the latest advances . '. Overall the book provides essential reading covering the recent advances, utilising molecular biology approaches, to further our understanding of fungal pathogens of humans. Despite the high price it would be a valuable addition to collections, and recommended reading for those with an interest in the molecular biology of human pathogenic fungi." from Expert Review of Anti-infective Therapy (2008) 6(5): 591-592.

"This new volume on the current research on fungal pathogens is a valuable resource for both scientists and clinicians. These discoveries will clearly lead to new drug discoveries and therapeutic tests that will save the lives of many patients." from Doodys (2008)

" . information of interest to a broad range of microbiologists/mycologists . a comprehensive overview of a number of rapidly evolving fields in molecular medical mycology. Overall, the book will provide a valuable resource for clinical research laboratories." from Microbiology Today (2009)

"This new volume on the current research on fungal pathogens is a valuable resource for both scientists and clinicians. These discoveries will clearly lead to new drug discoveries and therapeutic tests that will save the lives of many patients." from Shock (2008) 30: 753.

"the authors have done a good job in bringing together disparate references pertaining to a fascinating but technically involved area of study" from Australian Journal of Medical Science November (2010) 31: 4.

(EAN: 9781904455325 9781913652135 Subjects: [microbiology] [medical microbiology] [molecular microbiology] [mycology] )


Background

Algae consist of an extremely diverse, polyphyletic group of eukaryotic photosynthetic organisms. To characterize the genetic and metabolic diversity of chlorophytes (eukaryotic green algae) and to better understand how this diversity reflects adaptation to different habitats, we sequenced the trebouxiophyceaen Coccomyxa subellipsoidea C-169 NIES 2166. C-169 is a small elongated non-motile unicellular green alga (cell size of approximately 3 to 9 μm Figure S1A in Additional file 1) isolated in the polar summer of 1959/60 at Marble Point, Antarctica, from dried algal peat [1]. The Antarctic is a particularly harsh environment, with extremely low temperatures (as low as -88°C), frequent and rapid fluctuations from freezing to thawing temperatures, severe winds, low atmospheric humidity, and alternating long periods of sunlight and darkness. C-169 is psychrotolerant with an optimal temperature for growth at around 20°C in comparison, psychrophiles and psychrototrophs are organisms that have optimal growth temperatures of < 15°C and > 15°C, respectively, and a maximum growth temperature of < 20°C. C-169 was originally classified as Chlorella vulgaris, but present sequence data led to re-classification of the alga into the Coccomyxa genus with a species name of C. subellipsoidae (Supplemental Results in Additional file 2 and Figure S1 in Additional file 1).

C. subellipsoidea strains were first isolated in England and Ireland, where they form jelly-like incrustations on mosses and rocks [2, 3]. In contrast to its most closely sequenced relative, the trebouxiophyte Chlorella variabilis NC64A [4], which is an endosymbiont of paramecia, C-169 is free living. However, the type strain C. subellipsoidea SAG 216-13 as well as other isolates in the same species are known to form lichens with subarctic basidiomycetes of the genus Omphalina [5] other Coccomyxa spp. are intracellular symbionts of Ginkgo [6] and Stentors amethystinus [7] and intracellular parasites of mussels [8]. In the past 20 years C-169 has been used as a model organism in pioneering studies on green algal chromosome architecture. For example, early studies indicated that approximately 1.5% of its genome consists of LINE- and SINE-type retrotransposons [9, 10]. Additional studies provided a detailed analysis of the smallest 980 kb chromosome [11, 12].

Here we report the gene content, genome organization, and deduced metabolic capacity of C-169 and compare those features to other sequenced chlorophytes. We show that the C-169 gene repertoire encodes enzymatic functions not present in other sequenced green algae that are likely to represent hallmarks of its adaptation to the polar habitat.


Structure and function of the soil microbial community in a long-term fertilizer experiment

The effect of organic and inorganic fertiliser amendments is often studied shortly after addition of a single dose to the soil but less is known about the long-term effects of amendments. We conducted a study to determine the effects of long-term addition of organic and inorganic fertiliser amendments at low rates on soil chemical and biological properties. Surface soil samples were taken from an experimental field site near Cologne, Germany in summer 2000. At this site, five different treatments were established in 1969: mineral fertiliser (NPK), crop residues removed (mineral only) mineral fertiliser with crop residues manure 5.2 t ha −1 yr −1 sewage sludge 7.6 t ha −1 yr −1 or straw 4.0 t ha −1 yr −1 with 10 kg N as CaCN2 t straw −1 . The organic amendments increased the Corg content of the soil but had no significant effect on the dissolved organic C (DOC) content. The C/N ratio was highest in the straw treatment and lowest in the mineral only treatment. Of the enzymes studied, only protease activity was affected by the different amendments. It was highest after sewage amendment and lowest in the mineral only treatment. The ratios of Gram+ to Gram− bacteria and of bacteria to fungi, as determined by signature phospholipid fatty acids, were higher in the organic treatments than in the inorganic treatments. The community structure of bacteria and eukaryotic microorganisms was assessed by denaturing gradient gel electrophoresis (DGGE) and redundancy discriminate analyses of the DGGE banding patterns. While the bacterial community structure was affected by the treatments this was not the case for the eukaryotes. Bacterial and eukaryotic community structures were significantly affected by Corg content and C/N ratio.


Fungal-to-bacterial ratios in soils investigated for enhanced C sequestration

Fungi and bacteria govern most of the transformations and ensuing long-term storage of organic C in soils. We assessed the relative contributions of these two groups of organisms to the microbial biomass and activity of soils from five different ecosystems with treatments hypothesized to enhance soil C sequestration: (1) desert (an elevation gradient allowed comparison of soil developed in a cooler, moister climate with soil developed in a warmer, drier climate), (2) restored tallgrass prairie (land reverted to native prairie in 1979 and neighboring land farmed to row crops for ∼100 year), (3,4) two forest types (Douglas fir and loblolly pine, unfertilized control and N-fertilized plots), and (5) agricultural land (conventional- and no-till management systems). The selective inhibition technique, using captan (fungicide) and oxytetracycline hydrochloride (bactericide), was used to determine the activities (respiration) of fungi and bacteria in each of these soils and substrate-induced respiration was used to measure total active soil microbial biomass C. Phospholipid fatty acid analysis was used to determine the composition of the soil microbial biomass and determine if the activities and structure of the microbial communities were related. Differences in fungal-to-bacterial (F:B) activities between treatments at a site were greatest at the prairie sites. The restored prairie had the highest F:B (13.5) and high total C (49.9 g C kg −1 soil) neighboring soil farmed to corn had an F:B of 0.85 and total C of 36.0 g C kg −1 soil. Within the pairs of study soils, those that were tilled had lower fungal activities and stored C than those that were managed to native or no-till systems. In all pairs of soils, soils that had higher absolute fungal activities also had more total soil C and when two extreme cases were removed fungal activity was correlated with total soil C (R 2 =0.85). Thus, in this small set of diverse soils, increased fungal activities, more than F:B ratios, were associated with increased soil C. Practices that involved invasive land management decreased fungal activity and stored soil C compared to similar soils that were less intrusively managed.


Fungal Pathogen Biology

Ballou ER. 2020. mSphere of Influence: Positive Research Culture Enables Excellence and Innovation. mSphere 5:e00948-19.

Ballou ER, Gaffen SL, Gow NARG, Hise AG. 2019. Empowering Women: Moving from Awareness to Action at the Immunology of Fungal Infections Gordon Research Conference. Pathogens 8(3), 103.

Probert M, Zhou X, Goodall M, Johnston SA, Bielska E, Ballou ER, May, R.C. 2019. A Glucuronoxylomannan Epitope Exhibits Serotype-Specific Accessibility and Redistributes towards the Capsule Surface during Titanization of the Fungal Pathogen Cryptococcus neoformans. Infect Immun. 87(4).

Sephton-Clark PCS, Munoz JF, Ballou ER, Cuomo CA, Voelz K. 2018. Pathways of Pathogenicity: Transcriptional Stages of Germination in the Fatal Fungal Pathogen Rhizopus delemar. mSphere. 3(5)

Zhou X, Ballou ER. 2018. The Cryptococcus neoformans Titan Cell: From In Vivo Phenomenon to In Vitro Model. Current Clinical Microbiology Reports. 5(4):252-60.


Asian Journal of Biology

A survey was conducted during June to November, 2017 in five selected parks and gardens of Dhaka city, Bangladesh namely National Botanical Garden, National Zoo, Romna Park, Dhanmondi Lake and Boldha Garden. The investigation was done to analyze the morphology, diversity and distribution of macro fungi A total of 44 macro fungi samples were collected and identified to 32 species under 18 genera and 18 families. The most frequent collected genera were Ganoderma sp., Daedeleopsis sp., Ramariopsis sp., Crepidotus sp. and Daldinia sp. The maximum frequency of identified species was exhibited by Ganoderma lucidum (9.46%), followed by Ganoderma applanatum (8.1%), Volvariella volvacea (5.41%), Agaricus bisporus (5.41%) Daedaleopsis confragosa (4.05%), Trametes versicolor (4.05%) and Ganoderma boninense (4.05%). The maximum density of occurrence among collected samples was exhibited by Ramariopsis kunzei (11.3%), Ganoderma lucidum (9.9%), Crepidotus variabilis (5.3%) and Daedaleopsis confragosa (3.76%). The predominant species found in National Botanical Garden is Ganoderma applanatum, in Ramna Park is Ganoderma lucidum, in Dhanmondi Lake is Ramariopsis kunzei, in Boldha Garden is Ganoderma lucidum and in National zoo is Amanita bisporigera. The collected specimens were deposited to the Sher-e-Bangla Agricultural University Herbarium of Macro Fungi (SHMF).

  • Macro fungi
  • frequency
  • density
  • diversity
  • Ganoderma
  • Agaricus
  • Dhaka
  • Bangladesh.

How to Cite

References

Shaw CG, Kile GA. Armillaria root disease. Agriculture Handbook No.691. Forest Service, United States Department of Agriculture. Washington, D.C. 1991233.

Cheung PC. Mushrooms as functional foods. Food Science and Technology. 2008268.

Das K. Diversity and conservation of wild mushrooms in Sikkim with special reference to barsey rhododendron sanctuary. Botanical Survey of India, Sikkim Himalayan Regional Centre P. O. Rajbhawan, Gangtok - 737103, Sikkim, India. NeBIO. 20101(2):1-1.

Miles P, Chang ST. Mushrooms: Cultivation, nutritional value, medicinal effect and environmental impact. CRC, Boca Raton, FL. 2004451.

Chopra CM. Common edible fungi. Bugens publishing company, India. 1933223-230.

Purakasthya RP, Chandra A. Manual of Indian edible mushrooms. Today and tomorrow’s Publication, New Delhi 1985.

Rashid MH, Akhter K, Chowdhury MSM, Aminuzzaman FM. Biodiversity, habitat and morphology of mushroom of different forest regions of Bangladesh. Inter J Advan Res. 20175(9):945-957.

Barros BAV, Baptista P, Estevinho LM, Ferreira ICFR. Chemical composition and biological properties of Portuguese wild mushrooms: A Comprehensive Study. J Agric Food Chem. 200856:3856–3862.

Wani H, Pala SA, Boda RH, Mir RA. Morels in Southern Kashmir Himalaya. J. Mycol Pl Pathol. 201040:540-546.

Marjana A, Aminuzzaman FM, Chowdhury MSM, Mohsin SM, Das K. Diversity and ecology of macrofungi in Rangamati of Chittagong Hill Tracts under tropical evergreen and semi-evergreen forest of Bangladesh. Advan Res. 201813(5):1- 17.

Dickinson C, Lucas J. VNR Color Dictionary of Macrofungi. New York, New York: Van Nostrand Reinhold.198229.

Jorden P. The Macrofungi Guide and Identifier. Anness publishing limited Hermes house London 2000.

Pegler D, Spooner B. The Macrofungi Identifier. Quintet publishing limited 1997.

Zoberi MH. Some edible mushrooms from Nigeria. Nigerian Field.197338:81-90.

Das K, Aminuzzaman FM. Morphological and ecological characterization of xylotrophic fungi in mangrove forest region of Bangladesh. J Advan Biol Biot. 201711(4):1-15.

Rumainul MI, Aminuzzaman FM. Macro fungi biodiversity at the central and northern biosphere reserved areas of tropical moist deciduous forest region of Bangladesh. J Agric Ecol Res. 20165(4): 1-11.

Pushpa H, Purushothama KB. Biodiversity of mushrooms in and around Bangalore (Karnataka), India. American-Eurasian J Agric Environ Sci. 201212(6):750-759.

Kirk PM, Cannon PF, Minter DW, Stalpers JA. Dictionary of the Fungi (10th ed). Wallingford, UK: CAB International. 2008695.

Thiribhuvanamala G, Prakasam V, Chandrasekar G, Sakthivel K, Veeralakshmi S, Velazhahan R, Kalaiselvi G. Biodiversity, conservation and utilization of mushroom flora from the Western Ghats region of India. Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products (ICMBMP7), Tamil Nadu, India. 2011155-164.

Farooq M, Akram A, Afzal R, Nazir. Ethno-morphological studies of mushrooms collected from Soon Valley. J Phar Biol Sci. 20188(5):5-11.

Rubina H, Aminuzzaman FM, Chowdhury MSM, Das K. Morphological characterization of macro fungi associated with forest tree of National Botanical Garden, Dhaka. J Advan Biol & Biot. 201711(4):1-18.

Rumainul MI, Aminuzzaman FM, Chowdhury MSM. Biodiversity and morphological characterization of mushrooms at the tropical moist deciduous forest region of Bangladesh. American J Expt Agric. 20158(4):235-252.

Rashid, SN, Aminuzzaman FM, Islam MR, Rahman M, Rumainul MI. Biodiversity and distribution of wild mushrooms in the southern region of Bangladesh. J Advan Biol Biot. 20169(1):1-25.

Chandulal K, Gopal C, Priya J. Studies on biodiversity of fleshy fungi in Navsari (South Gujarat), India. Inter J Biodiversity and Conservation. 20135(8):508-514.

Buchalo AS, Solomon PW, Oksana BM, Viktor TB, Margarital L. Taxononical significance of mycrostructures in pure cultures of macromycetes. Proc Int Confer on Mushroom Biology and Mushroom Products 2011.

Aminuzzaman FM, Das K. Morphological characterization of polypore macro fungi associated with Dalbergia sissoo collected from Bogra district under social forest region of Bangladesh. J Biol Nat. 20176(4):199-212.

Rashid MH, Akhter K, Chowdhury MSM, Aminuzzaman FM. Biodiversity, habitat and morphology of mushroom of different forest regions of Bangladesh. Inter J Advan Res. 20175(9):945-957.

Tanjim A, Aminuzzaman FM, Rahaman M, Tanni JF. Biodiversity, distribution and morphological characterization of macro fungi in Sylhet and Moulvibazar under tropical evergreen and semi-evergreen forest regions of Bangladesh. Int J Adv Res. 20197(11):567-589.

Wang XC, Xi RJ, Li Y, Wang DM, Yao YJ. The species identity of the widely cultivated Ganoderma, ‘G. lucidum’ (Ling-zhi), in China. Plos One. 20127(7):408-571.

Dwivedi S, Tiwari MK, Chauhan UK, Panday AK. Biodiversity of mushrooms of Amarkantak Biosphere Reserve forest of Central India. International J Phar Life Sci. 20123(1):1363-1367.

Ram RC, Pandey VN, Singh HB. Morphological characterization of edible fleshy fungi from different forest regions. Indian J Sci Res. 20101(2):33-35.

Rahaman M, Aminuzzaman FM, Hossain MB, Rashid SN, Rumainul MI. Biodiversity, distribution and morphology of mushrooms of south western region of Bangladesh. J of Advan Res and Tech. 20164(3):60-79.

Mohanan C. Macrofungi of Kerala. Kerala, India: Kerala forest Research Institute. 2011597.

Andrew C, Heegaard E, Halvorsen R, Martinez-Peña F, Egli S, Kirk PM, Bässler C, Büntgen U, Aldea J, Høiland K, Boddy L. Climate impacts on fungal community and trait dynamics. Fungal Ecology 201622:17-25.

Bunyard AB. A survey of fungal diversity in Northeast Ohio. The Ohio Journal of Science. 2003103(2):29-32.

Smith AH. The North American Species of Naemalotoma. Mycologia.195143(5): 467.

Das K, Aminuzzaman FM, Akhtar N. Diversity of fleshy macro fungi in Mangrove forest regions of Bangladesh. Journal of Biology and Nature. 20176(4):218-241.

Ripkova S, Glejdura S. Crepidotus ehrendorferi in Slovakia and taxo-nomic notes on related species. Czech Mycol. 201061(2):175–185.

Bandala VM, Montoya L, Mata M. New species and records of Crepidotus from Costa Rica and Mexico. Fungal Diversity. 200832:9-29.

Eggert C, LaFayette PR, Temp U, Eriksson KE, Dean JF. Molecular analysis of a laccase gene from the White rot fungus. (Pycnoporus cinnabarinus). Appl Environ Microbiol. 199864:1766-72.

Chater AO, Brummitt RK. Subspecies in the works of Lepiota atrodisca.Taxon 1966.

Rajesh K, Ashwani T, Shailesh P, Rajib K, Devapod B, Jayasree B. Macro-fungal diversity and nutrient content of some edible mushrooms of Nagaland, india. Nusantara Bioscience. 20135(1):1-7.

Junior NM, Asai T, Capelari M, Paccola-Meirelles LD. Morphological and molecular identification of four Brazilian commercial isolates of Pleurotus spp. and cultivation on corn cob. Brazilian Arc Biol Tech. 2010 53(2):397-408.

Antonín V, Buyck B. Marasmius (Basidiomycota, Marasmiaceae) in Madagascar and the Mascarenes. Fungal Diversity. 200623:17-50.

Hosen MI, Li TH, Deng WQ.Amanita cinereovelata, a new species of amanita section lepidella from Bangladesh. Mycol Progress. 201514:35-37.

Lee HT, Nuytinck J, Verbeken A, Lumyong S, Desjardin D. Lactarius in northernspores and refractive hyphae in Amanita. Mycotaxon. 200752:305-396.


Asian Journal of Biology

A survey was conducted during June to November, 2017 in five selected parks and gardens of Dhaka city, Bangladesh namely National Botanical Garden, National Zoo, Romna Park, Dhanmondi Lake and Boldha Garden. The investigation was done to analyze the morphology, diversity and distribution of macro fungi A total of 44 macro fungi samples were collected and identified to 32 species under 18 genera and 18 families. The most frequent collected genera were Ganoderma sp., Daedeleopsis sp., Ramariopsis sp., Crepidotus sp. and Daldinia sp. The maximum frequency of identified species was exhibited by Ganoderma lucidum (9.46%), followed by Ganoderma applanatum (8.1%), Volvariella volvacea (5.41%), Agaricus bisporus (5.41%) Daedaleopsis confragosa (4.05%), Trametes versicolor (4.05%) and Ganoderma boninense (4.05%). The maximum density of occurrence among collected samples was exhibited by Ramariopsis kunzei (11.3%), Ganoderma lucidum (9.9%), Crepidotus variabilis (5.3%) and Daedaleopsis confragosa (3.76%). The predominant species found in National Botanical Garden is Ganoderma applanatum, in Ramna Park is Ganoderma lucidum, in Dhanmondi Lake is Ramariopsis kunzei, in Boldha Garden is Ganoderma lucidum and in National zoo is Amanita bisporigera. The collected specimens were deposited to the Sher-e-Bangla Agricultural University Herbarium of Macro Fungi (SHMF).

  • Macro fungi
  • frequency
  • density
  • diversity
  • Ganoderma
  • Agaricus
  • Dhaka
  • Bangladesh.

How to Cite

References

Shaw CG, Kile GA. Armillaria root disease. Agriculture Handbook No.691. Forest Service, United States Department of Agriculture. Washington, D.C. 1991233.

Cheung PC. Mushrooms as functional foods. Food Science and Technology. 2008268.

Das K. Diversity and conservation of wild mushrooms in Sikkim with special reference to barsey rhododendron sanctuary. Botanical Survey of India, Sikkim Himalayan Regional Centre P. O. Rajbhawan, Gangtok - 737103, Sikkim, India. NeBIO. 20101(2):1-1.

Miles P, Chang ST. Mushrooms: Cultivation, nutritional value, medicinal effect and environmental impact. CRC, Boca Raton, FL. 2004451.

Chopra CM. Common edible fungi. Bugens publishing company, India. 1933223-230.

Purakasthya RP, Chandra A. Manual of Indian edible mushrooms. Today and tomorrow’s Publication, New Delhi 1985.

Rashid MH, Akhter K, Chowdhury MSM, Aminuzzaman FM. Biodiversity, habitat and morphology of mushroom of different forest regions of Bangladesh. Inter J Advan Res. 20175(9):945-957.

Barros BAV, Baptista P, Estevinho LM, Ferreira ICFR. Chemical composition and biological properties of Portuguese wild mushrooms: A Comprehensive Study. J Agric Food Chem. 200856:3856–3862.

Wani H, Pala SA, Boda RH, Mir RA. Morels in Southern Kashmir Himalaya. J. Mycol Pl Pathol. 201040:540-546.

Marjana A, Aminuzzaman FM, Chowdhury MSM, Mohsin SM, Das K. Diversity and ecology of macrofungi in Rangamati of Chittagong Hill Tracts under tropical evergreen and semi-evergreen forest of Bangladesh. Advan Res. 201813(5):1- 17.

Dickinson C, Lucas J. VNR Color Dictionary of Macrofungi. New York, New York: Van Nostrand Reinhold.198229.

Jorden P. The Macrofungi Guide and Identifier. Anness publishing limited Hermes house London 2000.

Pegler D, Spooner B. The Macrofungi Identifier. Quintet publishing limited 1997.

Zoberi MH. Some edible mushrooms from Nigeria. Nigerian Field.197338:81-90.

Das K, Aminuzzaman FM. Morphological and ecological characterization of xylotrophic fungi in mangrove forest region of Bangladesh. J Advan Biol Biot. 201711(4):1-15.

Rumainul MI, Aminuzzaman FM. Macro fungi biodiversity at the central and northern biosphere reserved areas of tropical moist deciduous forest region of Bangladesh. J Agric Ecol Res. 20165(4): 1-11.

Pushpa H, Purushothama KB. Biodiversity of mushrooms in and around Bangalore (Karnataka), India. American-Eurasian J Agric Environ Sci. 201212(6):750-759.

Kirk PM, Cannon PF, Minter DW, Stalpers JA. Dictionary of the Fungi (10th ed). Wallingford, UK: CAB International. 2008695.

Thiribhuvanamala G, Prakasam V, Chandrasekar G, Sakthivel K, Veeralakshmi S, Velazhahan R, Kalaiselvi G. Biodiversity, conservation and utilization of mushroom flora from the Western Ghats region of India. Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products (ICMBMP7), Tamil Nadu, India. 2011155-164.

Farooq M, Akram A, Afzal R, Nazir. Ethno-morphological studies of mushrooms collected from Soon Valley. J Phar Biol Sci. 20188(5):5-11.

Rubina H, Aminuzzaman FM, Chowdhury MSM, Das K. Morphological characterization of macro fungi associated with forest tree of National Botanical Garden, Dhaka. J Advan Biol & Biot. 201711(4):1-18.

Rumainul MI, Aminuzzaman FM, Chowdhury MSM. Biodiversity and morphological characterization of mushrooms at the tropical moist deciduous forest region of Bangladesh. American J Expt Agric. 20158(4):235-252.

Rashid, SN, Aminuzzaman FM, Islam MR, Rahman M, Rumainul MI. Biodiversity and distribution of wild mushrooms in the southern region of Bangladesh. J Advan Biol Biot. 20169(1):1-25.

Chandulal K, Gopal C, Priya J. Studies on biodiversity of fleshy fungi in Navsari (South Gujarat), India. Inter J Biodiversity and Conservation. 20135(8):508-514.

Buchalo AS, Solomon PW, Oksana BM, Viktor TB, Margarital L. Taxononical significance of mycrostructures in pure cultures of macromycetes. Proc Int Confer on Mushroom Biology and Mushroom Products 2011.

Aminuzzaman FM, Das K. Morphological characterization of polypore macro fungi associated with Dalbergia sissoo collected from Bogra district under social forest region of Bangladesh. J Biol Nat. 20176(4):199-212.

Rashid MH, Akhter K, Chowdhury MSM, Aminuzzaman FM. Biodiversity, habitat and morphology of mushroom of different forest regions of Bangladesh. Inter J Advan Res. 20175(9):945-957.

Tanjim A, Aminuzzaman FM, Rahaman M, Tanni JF. Biodiversity, distribution and morphological characterization of macro fungi in Sylhet and Moulvibazar under tropical evergreen and semi-evergreen forest regions of Bangladesh. Int J Adv Res. 20197(11):567-589.

Wang XC, Xi RJ, Li Y, Wang DM, Yao YJ. The species identity of the widely cultivated Ganoderma, ‘G. lucidum’ (Ling-zhi), in China. Plos One. 20127(7):408-571.

Dwivedi S, Tiwari MK, Chauhan UK, Panday AK. Biodiversity of mushrooms of Amarkantak Biosphere Reserve forest of Central India. International J Phar Life Sci. 20123(1):1363-1367.

Ram RC, Pandey VN, Singh HB. Morphological characterization of edible fleshy fungi from different forest regions. Indian J Sci Res. 20101(2):33-35.

Rahaman M, Aminuzzaman FM, Hossain MB, Rashid SN, Rumainul MI. Biodiversity, distribution and morphology of mushrooms of south western region of Bangladesh. J of Advan Res and Tech. 20164(3):60-79.

Mohanan C. Macrofungi of Kerala. Kerala, India: Kerala forest Research Institute. 2011597.

Andrew C, Heegaard E, Halvorsen R, Martinez-Peña F, Egli S, Kirk PM, Bässler C, Büntgen U, Aldea J, Høiland K, Boddy L. Climate impacts on fungal community and trait dynamics. Fungal Ecology 201622:17-25.

Bunyard AB. A survey of fungal diversity in Northeast Ohio. The Ohio Journal of Science. 2003103(2):29-32.

Smith AH. The North American Species of Naemalotoma. Mycologia.195143(5): 467.

Das K, Aminuzzaman FM, Akhtar N. Diversity of fleshy macro fungi in Mangrove forest regions of Bangladesh. Journal of Biology and Nature. 20176(4):218-241.

Ripkova S, Glejdura S. Crepidotus ehrendorferi in Slovakia and taxo-nomic notes on related species. Czech Mycol. 201061(2):175–185.

Bandala VM, Montoya L, Mata M. New species and records of Crepidotus from Costa Rica and Mexico. Fungal Diversity. 200832:9-29.

Eggert C, LaFayette PR, Temp U, Eriksson KE, Dean JF. Molecular analysis of a laccase gene from the White rot fungus. (Pycnoporus cinnabarinus). Appl Environ Microbiol. 199864:1766-72.

Chater AO, Brummitt RK. Subspecies in the works of Lepiota atrodisca.Taxon 1966.

Rajesh K, Ashwani T, Shailesh P, Rajib K, Devapod B, Jayasree B. Macro-fungal diversity and nutrient content of some edible mushrooms of Nagaland, india. Nusantara Bioscience. 20135(1):1-7.

Junior NM, Asai T, Capelari M, Paccola-Meirelles LD. Morphological and molecular identification of four Brazilian commercial isolates of Pleurotus spp. and cultivation on corn cob. Brazilian Arc Biol Tech. 2010 53(2):397-408.

Antonín V, Buyck B. Marasmius (Basidiomycota, Marasmiaceae) in Madagascar and the Mascarenes. Fungal Diversity. 200623:17-50.

Hosen MI, Li TH, Deng WQ.Amanita cinereovelata, a new species of amanita section lepidella from Bangladesh. Mycol Progress. 201514:35-37.

Lee HT, Nuytinck J, Verbeken A, Lumyong S, Desjardin D. Lactarius in northernspores and refractive hyphae in Amanita. Mycotaxon. 200752:305-396.


UNIT 4: DIVERSITY OF LIFE

Chapter 9: Taxonomy and The World of Microorganisms and Viruses

Taxonomic Systems
Activity 9.1.1: Using a Classification Key
Viruses
Kingdom Monera
Investigation 9.3.1: Effects of Antiseptics
Kingdom Protista
Activity 9.4.1: Examining Protists

Chapter 10: Fungi and Plants

Kingdom-Fungi
Life Cycle of Fungi
Investigation 10.2.1: Monitoring Mould Growth
Importance of Fungi
Kingdom-Plantae
The Evolution of Terrestrial Plants
Alternation of Generations
Mosses
Ferns
Seed Plants

Chapter 10 Summary
Chapter 10 Review

Chapter 11: The Invertebrates

The Animal Kingdom
The Simplest Animals
The Worms
Activity 11.3.1: Earthworm Dissection
Mollusks and Echinoderms
The Arthropods

Chapter 11 Summary
Chapter 11 Review

Phylum Chordata
Fishes
Amphibians
Reptiles
Birds
Mammals
Activity 12.6.1: Using Models for Movement

Careers in Science
Chapter 12 Summary
Chapter 12 Review

Unit 4 Performance Task: Creating a Cladogram
Unit 4 Review


Abstract

Filamentous fungi are prolific producers of secondary metabolites with drug-like properties, and their genome sequences have revealed an untapped wealth of potential therapeutic leads. To better access these secondary metabolites and characterize their biosynthetic gene clusters, we applied a new platform for screening and heterologous expression of intact gene clusters that uses fungal artificial chromosomes and metabolomic scoring (FAC-MS). We leverage FAC-MS technology to identify the biosynthetic machinery responsible for production of acu-dioxomorpholine, a metabolite produced by the fungus, Aspergilllus aculeatus. The acu-dioxomorpholine nonribosomal peptide synthetase features a new type of condensation domain (designated CR) proposed to use a noncanonical arginine active site for ester bond formation. Using stable isotope labeling and MS, we determine that a phenyllactate monomer deriving from phenylalanine is incorporated into the diketomorpholine scaffold. Acu-dioxomorpholine is highly related to orphan inhibitors of P-glycoprotein targets in multidrug-resistant cancers, and identification of the biosynthetic pathway for this compound class enables genome mining for additional derivatives.


Watch the video: Pilze: Merkmale, Wachstum und Krankheiten Biologie. Duden Learnattack (July 2022).


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