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Why not use Cu(OH)2 as a dye for protein electrophoresis?

Why not use Cu(OH)2 as a dye for protein electrophoresis?


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As I know, $Cu(OH)_2$ $+$ Protein $ ightarrow$ Purple. So why not use $Cu(OH)_2$ as dye in protein electrophoresis?


Copper hydroxide does not dissolve very well in water, which means you will have trouble soaking them into your gels.

Also, even if you managed to add enough copper hydroxide beforehand (in the wells), it is unlikely that the copper(II) ions, if any existes, will travel along with the protein due to its size. (Copper hydroxide itself does not have any charge.)

As for the paper Stella mentioned, the copper salts are used after electrophoresis to produce a negative image, which is exactly what you cannot do with copper hydroxide.


  1. Protein electrophoresis is often done in an alkaline pH (8.6), which is not exactly nice if you want to use $Cu(OH)_2$.
  2. If you need quantitation, you can use Coumassie blue or Amido black. If you want sensitivity you can use silver. These existing stuff are quite OK.



Comments:

  1. Scelftun

    I think he is wrong. I'm sure. Let us try to discuss this. Write to me in PM, it talks to you.

  2. Aja

    I think he is wrong. I propose to discuss it. Write to me in PM, it talks to you.

  3. Jaymes

    I can't take part in the discussion right now - I'm very busy. I will be released - I will definitely express my opinion on this issue.



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